THE COMPLETE CRYOVIVA COLLECTION PROCESS
Collection Process of required samples in the delivery hospital by trained healthcare professionals
Maternal Blood: 3 tubes of maternal blood will be taken from mummy’s peripheral vein upon admission for delivery to test for infectious diseases.
Cord Blood: Cord blood is drawn by gravity into a collection bag after your baby is safely born and the cord has been clamped and cut. It is a safe and pain-free procedure to both mother and baby, which is less than five minutes.
Cord Tissue: Cord tissue is collected into a container immediately after cord blood collection is done. It is also a safe and pain-free procedure for both mother and baby, which is less than 2 minutes.
To all the expectant parents, all healthcare professionals in SG 10 maternity hospitals are trained by Cryoviva SG on the collection process.
Transportation of collected samples to Cryoviva SG Lab/ Reviva Cell Lab within 24 hours
Once you have delivered and required samples are collected by hospital professionals, please call our 24-hour hotline (65) 8339 8482 to collect the kit box back to our lab. All collected samples will reach our laboratory within 24 hours.
Upon calling, please provide details as listed below and we will advise you accordingly:
a) Mother’s Name
b) NRIC/Passport Number
c) Delivery Hospital
d) Residential ward and bed number
e) Contact number
This shouldn’t be bought from the refrigerated section of the grocery store as there is a risk of it containing listeria. Meat spreads are only safe to eat if they’ve been heated until steaming hot.
Testing and processing of all collected samples:
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| Maternal Blood testing | Cord Blood Testing | |
|---|---|---|
| Hepatitis Bs Antigen
Anti-HBc Total Anti-HCV HTLV I and II antibody CMV IgG Antibody CMV IgM Antibody HIV Ag/Ab VD (Syphilis TP Ab) NAT Screening: HIV-1/2 NAT Screening: HCV NAT Screening: HBV DNA |
Parameters | Volume (pre-processing) |
| Total Nucleated Cells Count (TNCC) | ||
| Cell Viability (pre-freeze) | ||
| Sterility test | Blood Culture – Aerobic | |
| Blood Culture – Anaerobic | ||
| CD markers | Total Live CD34+ Count | |
| others | Nucleated RBC | |
| ABO/Rh | ||
| HbA2 | ||
| Haemoglobin F | ||
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| Umbilical Cord Tissue Testing | |||
|---|---|---|---|
| CT Standard | CT Premium | ||
| Parameters | Total Nucleated Cells (TNC) | Yes | yes |
| Cell Viability | Yes | Yes | |
| Colony Forming Unit (CFU) | During Retrieval | Yes | |
| Sterility Test | Bacterial Culture | Yes | Yes |
| Fungal Culture | Yes | Yes | |
| Mycoplasma | During Retrieval | Before Storage & During Retrieval | |
| Endotoxin | During Retrieval | Before Storage & During Retrieval | |
| CD Markers | CD34 | – | Yes |
| CD45 | – | Yes | |
| HLA-DR | – | Yes | |
| CD73 | Yes | Yes | |
| CD90 | Yes | Yes | |
| CD105 | Yes | Yes | |
| Differentiation Study | Osteogenic | – | Yes |
| Chondrogenic | – | Yes | |
| Islet Cells | – | Yes | |
| Microscope | WJ-MSC Image | Yes | Yes |
Processing of stored samples:
Processing of Cord Blood: After collection, cord blood is transported to the Cryoviva SG laboratory, where it is processed using Sepax®2 fully-automated system and then gradually frozen, minus one degree at a time. Processing of Cord Tissue: after collection, Cord tissue is transported to the Reviva Cell Laboratory (a subsidiary of Cryoviva SG), where it is processed using Explant Technique by Reviva Cell Technology and then gradually frozen, minus one degree at a time.
Storage of the qualified samples and generation of a formal medical report to clients:
I. Storage of the CB: Cord Blood will be stored in U.S. FDA approved cryogenic storage pouch, with 2 integrally attached segments and frozen stored in vapor phase liquid nitrogen tanks when it reaches the optimal temperature.
II. Storage of the CT: Cord Tissue will be stored in cryo-vials and frozen stored in vapor phase liquid nitrogen tanks when it reaches the optimal temperature.
III. Formal Medical report: The formal medical report will be sent to our clients within 6 to 8 weeks times after successful storage in Cryoviva SG.
For unsuccessful storage case, there will be free of charge to our enrolled clients, and Cryoviva SG will bear for all the charges, which can include admin, collection kit and other incurred fees during collection.
